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KMID : 0380220060390040412
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Journal of Biochemistry and Molecular Biology
2006 Volume.39 No. 4 p.412 ~ p.417
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Identification of Transmembrane Domain of a Membrane Associated Protein NS5 of Dendrolimus punctatus Cytoplasmic Polyhedrosis Virus
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Chen Wuguo
Zhang Jiamin
Dong Changjin
Yang Bo
Li Yanqiu
Liu Chuanfeng
Hu Yuanyang
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Abstract
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We examined the intracellular localization of NS5 protein of Dendrolimus punctatus cytoplasmic polyhedrosis virus (DpCPV) by expressing NS5-GFP fusion protein and proteins from deletion mutants of NS5 in baculovirus recombinant infected insect Spodoptera frugiperda (Sf-9) cells. It was found that the NS5 protein was present at the plasma membrane of the cells, and that the N-terminal portion of the protein played a key role in the localization. A transmembrane region was identified to be present in the N-terminal portion of the protein, and the detailed transmembrane domain (SQIHMVWVKSGLVFF, 57-71aa) of N-terminal portion of NS5 was further determined, which was accorded with the predicted results, these findings suggested that NS5 might have an important function in viral life cycle.
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KEYWORD
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Cytoplasmic polyhedrosis virus, Immuno-gold labeling, Immunofluorescent, Membrane association protein, NS5
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